Effect of Multilayer Termination on Nonspecific Protein Adsorption and Antifouling Activity of Alginate-Based Layer-by-Layer Coatings

Layer-by-layer (LbL) assembly is a versatile platform for applying coatings and studying the properties of promising compounds antifouling applications. Here, alginate-based LbL were fabricated by alternating deposition alginic acid chitosan or polyethylenimine to form multilayer coatings. Films prepared with either odd even bilayer numbers investigate if termination affects physicochemical properties, resistance against nonspecific adsorption (NSA) proteins, efficacy. The hydrophilic films, which characterized using spectroscopic ellipsometry, water contact angle goniometry, ATR-FTIR spectroscopy, AFM, XPS, SPR revealed high swelling in strongly reduced NSA proteins compared hydrophobic reference. While choice polycation was important protein coatings, mattered less. attachment diatoms settling barnacle cypris larvae good that controlled charge density films.